Competence in Bacillus subtilis Transformation System
نویسندگان
چکیده
منابع مشابه
Development of competence in the Bacillus subtilis transformation system.
Competence in Bacillus subtilis, assayed by the ability of cells to be transformed with bacterial deoxyribonucleic acid (DNA) or transfected by phage DNA, has been shown to occur in a single semisynthetic medium with peak activity occurring 3 hr after the cessation of logarithmic growth. No step-down conditions or culture manipulations were necessary for routine transfection of 1% of the popula...
متن کاملBiochemical Aspects of Competence in the Bacillus subtilis Transformation System
A lytic factor, associated with the cell wall, was fortuitously discovered during the course of the investigations described in the previous paper (1). In the pneumococcal transformation system (2-5) as well as in the Bacillus subtilis transformation system (6), the highly transformable strains possessed active autolytic enzymes. Consequently, studies were undertaken to investigate the relation...
متن کاملBiochemical Aspects of Competence in the Bacillus Subtilis Transformation System. I. Chemical Composition of Cell Walls.
Populations of Pneumococcus (1)) Haemophilus inJEuenxae (2)) and Bacillus subtilis (3, 4) develop the capacity to incorporate and to be modified genetically by deoxyribonucleic acid during the latter portion of the logarithmic phase of growth. In 1946 McCarty, Taylor, and Avery (5) suggested that this rapid development of sensitivity to transforming DNA might be related to a reversible alterati...
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Some variables in the Bacillus licheniformis transformation system, such as inoculum size, initial cell density in transformation medium, and time of optimal competence, have been explored, and a methodology has been developed to obtain greater reproducibility and maximal competence for transformation. The transformation system in B. licheniformis has been shown to be similar to that of B. subt...
متن کاملEpisodic selection and the maintenance of competence and natural transformation in Bacillus subtilis.
We present a new hypothesis for the selective pressures responsible for maintaining natural competence and transformation. Our hypothesis is based in part on the observation that in Bacillus subtilis, where transformation is widespread, competence is associated with periods of nongrowth in otherwise growing populations. As postulated for the phenomenon of persistence, the short-term fitness cos...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1968
ISSN: 0022-1287
DOI: 10.1099/00221287-51-1-85